"LAMP" which stands for Loop-mediated Isothermal Amplification is a simple, rapid, specific and cost-effective nucleic acid amplification method solely developed by Eiken Chemical Co., Ltd.
It is characterized by the use of 4 different primers specifically designed to recognize 6 distinct regions on the target gene and the reaction process proceeds at a constant temperature using strand displacement reaction. Amplification and detection of gene can be completed in a single step, by incubating the mixture of samples, primers, DNA polymerase with strand displacement activity and substrates at a constant temperature (about 65°C).
It provides high amplification efficiency, with DNA being amplified 10^9-10^10 times in 15-60 minutes. Because of its high specificity, the presence of amplified product can indicate the presence of target gene.
Design 4 types of primers based on the following 6 distinct regions of the target gene: the F3c, F2c and F1c regions at the 3' side and the B1, B2 and B3 regions at the 5' side.
For more information about primers, the priniciple of LAMP and technical information: Eiken Genome Site
The each primer region can be determined by using the PrimerExplore (a special software to design LAMP primers) after considering the base composition, GC contents and the formation of secondary structures. Tm value can be obtained by Nearest Neighbor method.
LAMP technology has been applied to a wide range of fields to detect disease-causing bacteria and viruses. See more information at Eiken Genome Site.
Legionella, Cryptosporidium, Giardia
Salmonella, Verotoxin-producing Escherichia coli, Escherichia coli O157, Listeria monocytogenes, Campylobacter, Norovirus, Alicyclobacillus acidoterrestris (AAT), Flavobacterium psychro-philum
Bovine Embryo Sexing
peptidyl-prolyl cis-trans isomerase C, koi herpesvirus (KHV)
Leishmania, Mycoplasma pneumonia, Bordetella pertussis, Legionella pneumonia, H1 pdm 2009 Influenza, Influenza A virus, Influenza A subtype H5, SARS, Legionella, Aspergillus, Herpes simple, West Nile Virus (WNV), SARS-CoV-2 (COVID-19)
To achieve UHC, Eiken Chemical contributes to realize proper and early detection of infectious diseases including tuberculosis, malaria and neglected tropical diseases (NTDs). LAMP products are designed for the resource limited setting use and TB LAMP is recommended by WHO.
Now it’s time to action for “No one left behind”.