2021.12.13

Lively workshop was held! ISACP-ASVCP 2021 Workshop on Acute Phase Proteins

ISACP-ASVCP virtual workshop on Acute Phase Proteins (APPs) was held with a success on October 30, 2021.

APPs in cat, dog, horse, cow, porcine, mouse, rabbits, wildlife, … The topics were ranged from the basic information on APPs of each species to application in veterinary clinical pathology, such as when and which method of APP testing should be used in diagnostics. In the workshop, a lively discussion was made among speakers and participants by using online chat.

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  • Workshop details:
  • 2021 ISACP-ASVCP Pre-Meeting Workshop
  •  “Understanding the Crucial Role of Acute Phase Proteins in Veterinary Clinical Pathology”
  • October 30, 2021, Conjunction with ASVCP/ACVP Meeting
  • Workshop Introduction >      ISACP Website >
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  • Eiken Chemical is honored to introduce VET-SAA as a workshop sponsor.
  • We will share the Short Summary Video of what we introduced to the workshop participants. Please contact us for the details.  SAA Inquiry Form >

 

 

VET-SAA FAQ

Several questions were given to us at the workshop.

 

  • Q. What is the measurement principle of VET-SAA?
  • The measurement principle is latex turbidimetric immunoassay (LTIA) which is generally used for measurement on automated biochemistry analyzers. The image below shows the flow of reaction:

LTIA is based on the principle of measuring the change of light passing through the agglutination. When the reagent and sample are mixed in the cuvette on analyzer (01), antibodies in the reagent cause an antigen-antibody reaction and aggregate into larger complexes with antigens in the sample (02). The light is emitted through the agglutination, and the amount of antigen is estimated by measuring the light absorbance on a biochemistry analyzer (03).

 

  • Q. What antibodies are used for VET SAA?
  • VET-SAA uses monoclonal antibodies that recognize common sites in amino acid sequences between four animal species, horse, cat, dog and cow. By using monoclonal antibodies, stable reactivity to antigens and minimal batch variation can be realized.
  • VET-SAA linearity and reproducibility to each species:

We also confirm the reactivity with the following species SAA:

  • *Unpublished species are our in-house data. Publication list >
  • *If you are working with a novel species, proper validation must be conducted.

 

 

  • Q. I need the parameters for the biochemistry analyzer in my laboratory.
  • Please refer to the following page for analyzers we have applied before.
  • https://www.eiken.co.jp/en/products/vet_saa/vetsaa_application/
  • For other analyzers, we can propose the detailed settings such as measurement method, sample and reagent volume, time course, etc., based on our experience. Please contact us for more information.

 

 

  • Q. Can VET-SAA be performed using 96-well plate reader in a lab setting?
  • In our experience, it can be used to determine if SAA concentrations is high or low, however, please contact each sales representative for details.

 

 

  • Q. What should I use for daily quality control?
  • We prepare low and high concentration quality control materials. We recommend quality management by  them before actual sample measurement every day. For other quality control methods, please follow your local regulations.

VET-SAA-QC-High

VET-SAA-QC-Low

  • Q. How many tests can be performed per 1 kit of reagent?
  • Max. 300-400 tests can be performed per 1 kit. However, it depends on analyzers.

 

 

  • Q. How can I determine dilution schemes for samples with high SAA concentrations?
  • We have worked with collaborators and in house to determine recommended dilution schemes as the table shows. Please use saline for dilution.

Especially equine SAA can be much higher, which may require retest many times. An example of retest rate is as follows (In-house data):

  •   - No dilution:  40%
  •   - 5-fold dilution:  31%
  •   - 15-fold dilution:  13%
  •   - 30-fold dilution:  1%

 

 

  • Q. What is the difference from conventional reagent, LZ-SAA?
  • LZ-SAA was developed and used for measurement of human SAA. In response to the growing demand from users at veterinary laboratories, VET-SAA has been confirmed to be highly responsive to a wide range of species such as horses, cats, dogs and cow. VET-SAA has greatly improved sample dilution linearity and lower limit of measurement.　Nevertheless, some other species’ SAA have higher reactivity with LZ-SAA. Please contact us for the details.
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  • Q. Where can I purchase VET-SAA?
  • Eiken has a network of authorized distributors: Australia, New Zealand, Sweden, Finland, Hungary, Belgium, UK, Germany. Besides the above area, we have experiences of direct sales, especially to the US. Please contact us about other regions.

 

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Contact us

  • Didn’t find what you are looking for?
  • Are you interested in using VET-SAA for research or routine practice?

  • Enter your request here: SAA Inquiry Form

Product

VET-SAA ‘Eiken’ Reagent

Immunoassay reagent for multispecies SAA. *Veterinary use only product

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