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VET-SAA ‘Eiken’ – Latex turbidmetric immunoassay kit for veterinary research use

VET-SAA ‘Eiken’ is a reagent for general clinical chemistry analyzers. SAA, one of the acute-phase proteins (APPs) reflecting inflammation and tissue damage, stands for serum amyloid A protein.


New publication of equine SAA – 19 December, 2019

Jacobsen, S., Vinther, A.M., Kjelgaard-Hansen, M., Nielsen, L.N. Validation of an equine serum amyloid A assay with an unusually broad working range. BMC Vet Res 15, 462 (2019). https://doi.org/10.1186/s12917-019-2211-3

“The assay was unique in its ability to measure SAA concentrations with acceptable reliability over an extreme concentration range. This is relevant in the equine species, where SAA concentrations may reach very high concentrations” (Jacobsen et al. 2019).


New publication of rabbit SAA – February, 2020

Lennox A, Asahi Y, Arheart K, Ichiyanagi T, Cray C. Preliminary evaluation of an immunoturbidimetric assay and lateral flow device for the measurement of serum amyloid A in rabbits. Journal of Exotic Pet Medicine. 33 (2020) 54-56. https://doi.org/10.1053/j.jepm.2020.02.015

“Reference laboratory testing for APPs allows for accurate measurement of SAA and CRP ... Additional studies may show that APPs can have value in the diagnosis and prognosis of diseases in rabbits.” (Lennox et al. 2020)


What is the features of measuring SAA concentration?

1. Inflammation response can be clearly detected.
SAA concentration increases more than 10 to 1000 times by bacterial infection, viral infection, stress, trauma, inflammation, surgery, tumors, autoimmune diseases and tissue necrosis [*1].

2. SAA concentration is considered to reflect healing condition.
Since the half-life of SAA is estimated that 24-48 hours, the level of SAA keeps significant level if there is no recovery, but it decreases quickly in response to treatment [*1][*2].

3. SAA is observed as one of the major APPs in many animal species.
Compared with other APPs such as CRP, fibrinogen, a1-acid glycoprotein or haptoglobin, SAA is classified into major APP that increases >10-fold during the response to inflammation in a wide range of species [*3]. Also, the amino acid sequence has high homology between animals [*4].

What is SAA?

Serum amyloid A (SAA) is an apolipoprotein associated in HDL and has 11.4 kDa molecular weight. [*4]
SAA is one of the major APPs that in many species. Cray et al. (2009) suggest that feline, bovine, canine, goat, equine, mouse, porcine, rabbit and sheep has SAA as major APP [*3]. Also, our predecessor reagent “SAA-1,” using the same antibodies as VET-SAA, reacts to SAA in species as follows: equine, canine, feline, ferret, rabbit, antelope, blackbuck, bongo, cheetah, deer, elephant seal, gerenuk, leopard, lion, manatee, rhesus monkey, tiger, walrus, white faced saki and zebra.

References:
*1 Jacobsen S, Andersen PH. The acute phase protein serum amyloid A (SAA) as a marker of inflammation in horses. EqVet Educ 2007;19:38–46.
*2 Tamamoto T, Ohno K, Ohmi A, Goto-Koshino Y, Tsujimoto H. Verification of measurement of the feline serum amyloid A (SAA) concentration by human SAA turbidimetric immunoassay and its clinical application. J Vet Med Sci 2008; 70:1247–1252.
*3 Cray C, Zaias J, Altman NH. Acute phase response in animals: a review. Comp. Med., 59 2009, pp. 517-526.
*4 Sack GH Jr. Serum amyloid A—a review. Mol Med (Cambridge, Mass) 2018;24:46.


VET-SAA ‘Eiken’– Product information

VET-SAA was developed to react to SAA in multi species. We chose optimal monoclonal antibodies which react animal SAA as horses, cows, dogs, cats and rabbits. We are looking for more investigation of application to other animal species. Please contact us if you are interested in collaborative research.

VET-SAA ‘Eiken’ Reagent VET-SAA Calibrator Set VET-SAA-QC-Low / High


Veterinary Research Use Only

Code No.

Product Name

Units

V-SZ51

VET-SAA ‘Eiken’ Reagent

R1: 20mL×2
R2: 20mL×2

V-SZ90

VET-SAA Calibrator Set

Calibrator 5x1 mL
Lysis buffer 1x12mL

V-SZ91

VET-SAA-QC-Low

Control 5x1 mL
Lysis buffer 1x12mL

V-SZ92

VET-SAA-QC-High

Control 5x1 mL
Lysis buffer 1x12mL



Analytical performance

VET-SAA 'Eiken' Analytical performance data [Eiken Chemical]



LZ Test 'EIKEN'

LZ Test is a reagent for general-purpose fully automated analyzers operating by latex
photometric immunoassay method. EIKEN CHEMICAL developed and supplies products such as reagents for tests of atrophic gastritis - Pepsinogen I and II - and H.pylori Antibody.

ABCD classification is useful for screening gastric cancer risks.
(Helicobacter pylori + Pepsinogen test)

- The number of unnecessary endoscopy is reduced by prioritizing patients based on the risk group of gastric cancer.
- The reduction of endoscopy examination to healthy people might contribute to decrease healthcare costs.

What is ABCD classification?

Gastric Cancer Risk Assessment Test (ABCD classification) is a method for determining the health status of the stomach of an individual by means of a combination of a pepsinogen test (as a gastric mucosal atrophy marker) and a Helicobacter pylori antibody test for screening H. pylori infections (considered to be the main cause of gastric ulcers, duodenal ulcers, and gastric cancers).

Pepsinogen (PG) test:
It is a blood test designed to objectively evaluate the health status (degree of mucosal atrophy) of the stomach by measuring the serum concentration of a substance called “pepsinogen”. Many gastric cancer cases are shown to develop subsequent to gastric mucosal atrophy, the extent of which can be estimated using the pepsinogen test, and the test results can be utilized to screen individuals for increased risk of gastric cancer.

H. pylori antibody test:
It is a screening test of H. pylori infections of the gastric mucosa. When infected with H. pylori, the body produces antibodies against H. pylori (H. pylori antibodies) which are released into the blood. This antibody test is a method for measuring these H. pylori antibodies in the blood of the individual.

LZ Test 'EIKEN'

IVD
Code No.

Product Name

Product Description

V-IY05 LZ H.pylori Antibody Reagent
- with CE-IVD-
For detection of Helicobacter pylori antibodies in serum or plasma.
V-IZ51 LZ-PEPSINOGENⅠ
- with CE-IVD-
For measurement of PepsinogenⅠ in serum or plasma.
V-IZ53 LZ-PEPSINOGENⅡ
- with CE-IVD-

For measurement of PepsinogenⅡ in serum or plasma.

We also provide other reagents for LZ Test ‘EIKEN’ series.
See the brochure