Loopamp™ SARS-CoV-2 Detection Kit

CE marked COVID-19 testing

Loopamp™ SARS-CoV-2 Detection Kit

The kit is an unique method of gene amplification that amplifies nucleic acid with high specificity, efficiency, and rapidity under isothermal condition. An one-step, single tube, reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detecting SARS-CoV-2.

Fighting against the COVID-19 global pandemic

In response to the COVID-19 pandemic around the world, Eiken Chemical has developed Loopamp™ SARS-CoV-2 Detection Kit to enable clinical and public health laboratories to quickly diagnose SARS-CoV-2 infection. 

To control and suppress spread, we must fight against COVID-19. Active case finding, diagnosis, isolation and contact tracing can control COVID-19. In particular, strengthening surveillance and contact tracing will help every authority to rapidly adapt to monitoring outbreaks.

We must continue to stand together to tackle the greatest public health challenge of the century. Our challenge is great. Together, forward in the fight against COVID-19. We believe our test Loopamp™ SARS-CoV-2 Detection Kit contributes to your health.

Characteristics of Loopamp™ SARS-CoV-2 Detection Kit

  1. Fast and simple: Extraction in 5 min and amplification in 35 min under isothermal condition at 62.5°C .
  2. Robust design: Simple instruments: Incubator or Thermal Cycler
  3. Easy operation: Dried amplification reagent and primer mix are ready to use
  4. Visual detection: No need for expensive and high complex equipment
  5. Experience:  Since its launch under the Japanese IVD regulation on April 9, 2020, it has been enhancing the quality of products, services and support.

Necessary equipment and labware

  1. Incubator, Thermal Cycler (Temperature accuracy ±0.5°C)
  2. Sterile cotton swab for collecting a sample
  3. Micro pipettes and filter tip (Primer mix: 15uL / Sample: 10uL)
  4. Aluminum rack for cooling and ice box (1.5 mL tube / 0.2 mL tube)
  5. Micro simple centrifuge (1.5 mL tube)
  6. Simple centrifuge for 8 micro tubes (0.2 mL tube)

 

Applicable instruments:

  1. Incubator
  2. Appropriate Real-Time PCR instrument

Loopamp™ SARS-CoV-2 Detection Kit was validated to be used with the following Real-Time PCR instruments.

  • - Applied Biosystem® 7500 Real-Time PCR System (software version 2.3)
  • - Roche® LightCycler 480 II (software version 1.5)
  • - Bio-Rad CFX Maestro (software version 1.1)

Other instruments with temperature accuracy  ±0.5°C are applicable to Loopamp™ SARS-CoV-2 Detection Kit.

Simple operation

Comparison of LAMP and PCR

  LAMP PCR / Real time PCR
Temperature Isothermal Thermal cycling
Time 35 minutes 2-3 hours
Specificity 4 primers for 6 regions

2 primers for 2 regions

Visual Detection Available Not available
Influences of inhibitors Robust Not robust
RNA Extraction Simple and easy Complicated

Contents of product

  • - Applicable sample: Nasopharyngeal, oropharyngeal swab samples and saliva samples
  • - CE marked
Product code Product name Contents Units Storage
LMP403 Loopamp™ SARS-CoV-2 Detection Kit

Dried RNA Amplification Reagent  (dRMR)

Primer Mix 2019-nCoV  (PM nCV19)

Positive Control 2019-nCoV (PC nCV19)

Negative Control (NC)

48 tests 2-8°C
LMP801 Loopamp™ RNA Viral Extraction Kit *1 Extraction Reagent 4 mL x 48 48 tests Room temperature

*1 Use Loopamp™ RNA Viral Extraction Kit to extract RNA in human-derived samples and use it as a sample solution

Publications

Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 2000 Jun 15;28(12):E63.   PubMed >
Kitagawa Y, Orihara Y, Kawamura R, et al. Evaluation of rapid diagnosis of novel coronavirus disease (COVID-19) using loop-mediated isothermal amplification. J Clin Virol. 2020 May 21;129:104446.   PubMed >
Nagura-Ikeda M, Imai K, Tabata S, et al. Clinical evaluation of self-collected saliva by RT-qPCR, direct RT-qPCR, RT-LAMP, and a rapid antigen test to diagnose COVID-19. J Clin Microbiol. 2020 Jul 7:JCM.01438-20.   PubMed >

 

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